Rapid seroconversion and persistent functional IgG antibodies in severe COVID-19 patients correlates with an IL-12p70 and IL-33 signature

Abstract Despite ongoing efforts to characterize the host response toward SARS-CoV-2, a major gap in our knowledge still exists regarding magnitude and duration of humoral response. Analysis antibody mild versus moderate/severe patients, using new developed quantitative electrochemiluminescent assay for detecting IgM/IgA/IgG antibodies SARS-CoV-2 antigens, revealed rapid onset IgG/IgA antibodies, specifically patients. IgM against viral receptor binding domain, but not nucleocapsid protein, were detected at early stages disease. Furthermore, we observed marked reduction IgM/IgA over-time. Adapting ACE2 binding-competition, demonstrated that presence potentially neutralizing is corelated with IgG/IgA. Finally, analysis cytokine profile COVID-19 patients unique correlation an IL-12p70/IL33 IgG seroconversion, which correlated disease severity. In summary, comprehensive has implications on understanding monitoring infections.